A DNA extraction method for RAPD analysis from plants rich in soluble polysaccharides |
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| Authors: | Qin-Bao Li Qinyin Cai Charles L Guy |
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| Institution: | (1) Program for Plant Molecular and Cellular Biology, Department of Environmental Horticulture, Institute of Food and Agricultural Science, University of Florida, 32611-0512 Gainesville, Florida, USA |
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| Abstract: | A simple procedure for the isolation of DNA from mature leaf tissue was developed. This procedure purified DNA using Sephacryl
S-1000 column and PEG 8000 precipitation. Polysaccharide-like components were successfully removed from DNA samples from species
in which polysaccharides were found to be difficult to remove by phenol/chloroform extraction. The DNA obtained by this method
was suitable for PCR, RAPD, enzyme digestion, and Southern-blot analyses. |
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| Keywords: | DNA isolation Sephacryl PEG precipitation |
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