Skin Tattooing As A Novel Approach For DNA Vaccine Delivery |
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Authors: | Yung-Nung Chiu Jared M Sampson Xunqing Jiang Susan B Zolla-Pazner Xiang-Peng Kong |
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Institution: | Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine;Department of Pathology, New York University School of Medicine;Healthcare System, Veterans Affairs New York Harbor |
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Abstract: | Nucleic acid-based vaccination is a topic of growing interest, especially plasmid DNA (pDNA) encoding immunologically important antigens. After the engineered pDNA is administered to the vaccines, it is transcribed and translated into immunogen proteins that can elicit responses from the immune system. Many ways of delivering DNA vaccines have been investigated; however each delivery route has its own advantages and pitfalls. Skin tattooing is a novel technique that is safe, cost-effective, and convenient. In addition, the punctures inflicted by the needle could also serve as a potent adjuvant. Here, we a) demonstrate the intradermal delivery of plasmid DNA encoding enhanced green fluorescent protein (pCX-EGFP) in a mouse model using a tattooing device and b) confirm the effective expression of EGFP in the skin cells using confocal microscopy. |
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Keywords: | Bioengineering Issue 68 Biomedical Engineering Genetics Medicine DNA vaccine immunization method skin tattooing intradermal delivery GFP |
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