Characterization of Bombyx mori nucleopolyhedrovirus with a knockout of Bm17 |
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| Authors: | Hongxing Shen Yang Zhou Wen Zhang Bin Nin Hua Wang Xiaochun Wang Shihe Shao Huiqing Chen Zhongjian Guo Xiaoyong Liu Qin Yao Keping Chen |
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| Institution: | 1.School of Medical Science and Laborarory Medicine, Jiangsu University, Zhenjiang, 212013 People’s Republic of China ;2.Institute of Life Sciences, Jiangsu University, 301# xuefu road, Zhenjiang, 212013 People’s Republic of China |
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| Abstract: | Open reading frame 17 (Bm17) gene of Bombyx mori nucleopolyhedrovirus is a highly conserved gene in lepidopteran nucleopolyhedroviruses, but its function remains unknown. In this report, transient-expression and superinfection assays indicated that BM17 localized in the nucleus and cytoplasm of infected BmN cells. To determine the role of Bm17 in baculovirus life cycle, we constructed a Bm17 knockout virus and characterized its properties in cells. Analysis of the production and infection of budded virions, the level of viral DNA replication revealed showed that there was no significant difference among the mutant, the control, and the Bm17 repaired virus strains. These results suggest that BM17 is not essential for virus replication in cultured cells. |
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| Keywords: | Baculovirus Bm17 BmNPV DNA virus Gene knock-out |
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