Advanced microscale bioreactor system: a representative scale-down model for bench-top bioreactors |
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Authors: | Wei-Ting Hsu Rigzen P S Aulakh Donald L Traul Inn H Yuk |
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Institution: | 1.Early Stage Cell Culture, Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080 USA ;2.TAP Biosystems, York Way, Royston, Hertfordshire SG8 5WY UK |
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Abstract: | In recent years, several automated scale-down bioreactor systems have been developed to increase efficiency in cell culture process development. ambr™ is an automated workstation that provides individual monitoring and control of culture dissolved oxygen and pH in single-use, stirred-tank bioreactors at a working volume of 10–15 mL. To evaluate the ambr™ system, we compared the performance of four recombinant Chinese hamster ovary cell lines in a fed-batch process in parallel ambr™, 2-L bench-top bioreactors, and shake flasks. Cultures in ambr™ matched 2-L bioreactors in controlling the environment (temperature, dissolved oxygen, and pH) and in culture performance (growth, viability, glucose, lactate, Na+, osmolality, titer, and product quality). However, cultures in shake flasks did not show comparable performance to the ambr™ and 2-L bioreactors. |
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Keywords: | Scale-down system Chinese hamster ovary Cell culture automation ambr™ Single-use bioreactor |
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