Cryopreservation of Trypanosoma cruzi Bloodstream Forms* |
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Authors: | LENY S. FILARDI Z. BRENER |
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Abstract: | SYNOPSIS. Recovery rates of T. cruzi bloodstream forms subjected to several methods of cryopreservation in liquid nitrogen and at -73 C are reported. Inoculations of animals with cryopreserved and nonpreserved trypomastigotes revealed that prolonged storage at -196 C apparently did not change the biologic characteristics of different T. cruzi strains. The reproducibility and consistency of results suggest that “cryobanks'’or “reference centers'’may be established. |
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Keywords: | Trypanosoma cruzi bloodstream forms cryopreservation liquid nitrogen effect on biologic characteristics |
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