| Abstract: | Circulating 14C]glucose 2, 5 and 10 min after intravenous injection of U-14C]-L-alanine was greater in 24 hr starved than in fed rats. In vitro uptake of 14C]alanine by liver and kidney cortex slices from 24 hr starved and fed rats rose in parallel with increased medium substrate concentration. Formation of 14C]glucose from 1mM 14C]alanine was similar in liver and kidney cortex slices and increased in tissues from 24 hr starved compared with fed rats. With 5 mM 14C]alanine more 14C]glucose was produced by liver than by kidney cortex slices from 24 hr starved rats. Liver slices always produced more 14C]lactate and less 14C]-CO2 from 14C]alanine than kidney cortex slices. It is proposed that under physiological conditions, the kidneys cortex actively participates in glucose production from alanine. |
