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Ultrastructure of Nitrosospira sp. X101 with crystalline outer membrane protein (COMP)
Authors:Pertti J. Martikainen   Eeva-Liisa Nurmiaho-Lassila  Kari Lounatmaa
Affiliation:Department of General Microbiology, University of Helsinki, Helsinki, Finland;Department of Electron Microscopy, University of Helsinki, Helsinki, Finland
Abstract:Abstract The outer membrane (OM) structure of Nitrosospira sp. X101 was studied by different electron microscopic techniques and SDS-PAGE. A crystalline outer membrane protein was visible in freeze-etched cells, occasionally seen also in the thin sectioned cells, but was difficult to see in a negatively-stained preparation. The lattice probably consists of large globular protein subunits with a hexagonal arrangement. The molecular weights of the major proteins in the cell envelope are 35 kDa, 40 kDa and 42 kDa.
Keywords:Soil bacterium    Nitrosospira    Ultrastructure    Crystalline outer membrane protein
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