Cellular and biochemical parameters of exercise-induced oxidative stress: relationship with training levels |
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Authors: | Pittaluga Monica Parisi Paolo Sabatini Stefania Ceci Roberta Caporossi Daniela Valeria Catani M Savini Isabella Avigliano Luciana |
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Affiliation: | a Laboratory of Biology and Biochemistry of Movement, Department of Sciences of Human Movement and Sport, IUSM—University of Sport and Movement Sciences, Rome, Italyb Department of Experimental Medicine & Biochemical Sciences, University of Rome Tor Vergata, Rome, Italy |
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Abstract: | To better clarify the relationship between physical activity and oxidative stress, we determined the effects of a maximal test in 18 young subjects with different training levels (six professional Athletes and 12 non-agonists (NA)). Redox homeostasis (total antioxidant activity (TAS), vitamin C and glutathione (GSH)), oxidative damage (diene conjugation and hemolysis), lymphocyte cell death and repair systems (apoptosis, micronuclei and Hsp70 expression) were evaluated. We found that agonistic training led to a chronic oxidative insult (high baseline values of oxidized glutathione (GSSG), micronuclei and hemolysis). On the contrary, NA with the lowest level of training frequency showed a well balanced profile at rest, but they were more susceptible to exercise-induced variations (GSSG/GSH and diene increased values), respect to the NA with an higher level of training. As almost all the parameters employed in this study showed inter-individual variations, the GSSG/GSH ratio remains the most sensitive and reliable marker of oxidative stress, accordingly with other data just reported in the literature. |
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Keywords: | Oxidative stress redox balance physical activity DNA damage apoptosis |
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