Genomic homology of the domestic ferret with cats and humans |
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Authors: | Pietro Cavagna Alessandra Menotti Roscoe Stanyon |
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Institution: | (1) Department of Experimental Biology, University of Genoa Viale Benedetto XV, 16132 Genoa, Italy, IT;(2) Basic Research Laboratory, Department of Anthropological Sciences, University of Genoa, Via Balbi 4, 16126 Genoa, Italy, IT;(3) Molecular Cytogenetic Section, National Cancer Institute, Bldg 560, Frederick, Maryland 21702, USA, US |
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Abstract: | We used chromosome paints from both the domestic cat and humans to directly establish chromosomal homology between the genome
of these species and the domestic ferret. The chromosome painting data indicate that the ferret has a highly conserved karyotype
closer to the ancestral carnivore karyotype than that of the cat. The cat chromosome paints revealed 22 homologous autosomal
regions in the ferret genome: 16 ferret chromosomes were hybridized by a single cat paint, while 3 ferret chromosomes were
hybridized by two cat paints. In situ hybridization combined with banding showed that ferret Chromosome (Chr) 1 = cat A2p/C2,
Chr 2 = F2/C1q, and Chr 3 = A2q/D2. Five ferret chromosomes are homologous to single arms of cat chromosomes: ferret 4 = A1q,
5 = B1q, 6 = C1p, 10 = A1p, and 12 = B1p. The human chromosome paints revealed 32 + XY homologous regions in the ferret genome:
9 ferret chromosomes were each hybridized by a single human paint, 7 by two paints, 3 by three paints. The 10 ferret chromosomes
hybridized by multiple human paints produced the following associations: ferret 1 = human 19/3/21, 2 = 8q/2q, 3 = 10/7, 5
= 8/4, 8 = 15/14, 9 = 10/12/22, 11 = 20/2, 12 = 8/4, 14 = 12/22/18, 18 = 19/16. We present an index of genomic diversity,
Z, based on the relative number of conserved whole chromosome and chromosome segments as a preliminary statistic for rapid
comparison between species. The index of diversity between human-ferret (Z = 0.812) is slightly less than human-cat (Z = 0.843).
The homology data presented here allow us to transfer gene mapping data from both cats and humans to the ferret.
Received: 21 December 1999 / Accepted: 30 May 2000 |
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