Novel mastoparan and protonectin analogs isolated from a solitary wasp, Orancistrocerus drewseni drewseni |
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Authors: | Murata Kazuya Shinada Tetsuro Ohfune Yasufumi Hisada Miki Yasuda Akikazu Naoki Hideo Nakajima Terumi |
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Institution: | (1) Graduate School of Science, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi-ku, Osaka 558-8585, Japan;(2) Suntory Institute for Bioorganic Research, 1-1-1 Wakayamadai Shimamoto-cho, Mishima-gun, Osaka 618-8503, Japan; |
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Abstract: | Two novel biologically active peptides, Eumenine mastoparan-OD and Orancis-Protonectin, were isolated from a solitary wasp,
Orancistrocerus drewseni
drewseni (Eumeninae, Vespidae). MALDI-TOF MS analysis of a small amount of the crude venom gave two intensive molecular-related ion
peaks at m/z 1269.9 and 1552.9 that were expected to be novel based on a peptide database search. Purification of the crude venom by HPLC
gave two peptide fractions, P-1 and P-2. The amino acid sequence of P-1 (GRILSFIKAGLAEHL-NH2) and P-2 (ILGIITSLLKSL-NH2) were determined by ESI-MS/MS, automated Edman degradation, and amino acid analysis. According to the high sequence homology
with those of mastoparan and protonectin, P-1 and P-2 were labeled Eumenine mastoparan-OD and Orancis-Protonectin, respectively.
Orancis-Protonectin is the first example of a protonectin analog isolated from the venom of a solitary wasp. The hemolytic
activities of these new peptides were more potent than that of mastoparan. |
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