Simple and rapid procedure for chromatographic identification of multiple transfer RNA species from Escherichia coli |
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Authors: | W D Graziadei W J Fimian D J Plocke |
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Affiliation: | Department of Biology, Boston College, Chestnut Hill, Massachusetts 02167 USA |
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Abstract: | A modification of the benzoylated DEAE-cellulose method of tRNA fractionation has been developed to provide a rapid and highly efficient method for the quantitative identification of multiple tryptophan, tyrosine, and phenylalanine tRNA species from E. coli aminoacylated in vivo. This method should be of particular use in physiological studies of these tRNA species. |
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