Fasciola hepatica: cytochrome c oxidoreductases and effects of oxygen tension and inhibitors |
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| Authors: | R K Prichard P J Schofield |
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| Institution: | Division of Animal Health, C.S.I.R.O., McMaster Laboratory, Glebe, N.S.W. 2037, Australia; School of Biochemistry, University of New South Wales, Kensington, N.S.W. 2033, Australia |
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| Abstract: | Studies were made on the mechanism of respiration in Fasciola hepatica (Trematoda). Respiration was found to be dependent on the oxygen tension. The respiratory enzyme systems, NADH-cytochrome c oxidoreductase (EC 1.6.2.1), succinate-cytochrome c oxidoreductase (EC 1.3.99.1) NADH oxidase and cytochrome c-oxygen oxidoreductase (EC 1.9.3.1) were detected in a mitochondrial preparation, the NADH oxidase activity being markedly stimulated by addition of mammalian cytochrome c. Amytal and rotenone inhibited NADH oxidase activity. Antimycin A inhibited succinoxidase activity only at relatively high concentrations. Azide was inhibitory at high concentrations. However, cyanide was found to stimulate respiration. Hydrogen peroxide was found to be an end product of respiration in F. hepatica. |
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| Keywords: | Fasciola hepatica Trematoda Respiration Enzymes NADH-cytochrome c oxidoreductase (EC 1 6 2 1) Succinate-cytochrome c oxidoreductase (EC 1 3 99 1) Cytochrome c-oxygen oxidoreductase (EC 1 9 3 1) NADH oxidase Inhibitors respiratory Hydrogen peroxide Metabolism Antimycin Rotenone Azide |
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