Cultured myometrial cells establish communicating gap junctions |
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| Authors: | R L Caruso D R Juberg V Caldwell I A Corcos |
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| Affiliation: | Department of Environmental and Industrial Health, University of Michigan School of Public Health, Ann Arbor 48109-2029. |
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| Abstract: | Myometrial cells were isolated and cultured from term rat uterus. The myometrial origin of the cultures was verified by antibody staining of cellular desmin and alpha-smooth muscle actin. The presence of functional gap junctions was indicated by transfer of radiolabeled nucleotide and microinjected Lucifer yellow dye. The cultured cells expressed mRNA recognized by a connexin43 gap junction cDNA probe. To our knowledge, this is the first report that isolated myometrial cells form gap junctions in culture. |
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