Autogenous growth factor production by reticuloendotheliosis virus-transformed hematopoietic cells |
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Authors: | R F Garry H R Bose |
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Affiliation: | Department of Microbiology and Immunology, Tulane University School of Medicine, New Orleans, Louisiana 70112. |
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Abstract: | Reticuloendotheliosis virus strain T (REV-T)-transformed cells gave rise spontaneously to variants which secrete a factor that forms a distinct visible ring of precipitation (halo) surrounding colonies grown in soft agar. An Mr 15,000 protein was produced at higher levels by halo variants than by nonhalo-producing cells. An assay designed to detect the formation of precipitates enabled purification of an Mr 15,000 protein, p15, from serum-free medium conditioned by the growth of REV-T-transformed hematopoietic cells. Fractions enriched in p15 permitted the growth of REV-T-transformed cells under conditions where they normally failed to proliferate. |
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Keywords: | retrovirus transformation v-rel lymphoid cell variants |
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