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Pituitary astrocytes from the neural lobe of rats
Authors:Stephen J. Bunn  Michael R. Hanley  Graham P. Wilkin
Affiliation:(1) Department of Biochemistry, Imperial College of Science and Technology, London, United Kingdom;(2) Present address: The Neuroscience Group, Faculty of Medicine, The University of Newcastle, 2308 Shortland, NSW, Australia;(3) Present address: The MRC Molecular Neurobiology Unit, MRC Centre, Hills Road, Cambridge, UK;(4) The Neuroscience Group, Faculty of Medicine, The University of Newcastle, 2308 Shortland, NSW, Australia
Abstract:Summary Tissue culture preparations of adult and neonatal rat pituitary neural lobes were examined by use of cell-type specific immunohistochemical markers. Cultures obtained from explanted or dissociated adult tissue or explanted neonatal tissue produced cells immunoreactive for endothelial and fibroblast markers. In contrast, dissociated neonatal tissue produced, in addition, two distinct forms of astrocytic glial cells immunoreactive for glial fibrillary acidic protein, one of which was also immunoreactive for the ganglioside GD3.
Keywords:Pituitary  Pituicytes  Glial fibrillary acidic protein  Astrocytes  Cell culture  Rat (Sprague-Dawley)
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