Identification of the basement membrane protein nidogen as a candidate ligand for tumor endothelial marker 7 in vitro and in vivo |
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Authors: | Lee Hyun Kyoung Seo In Ae Park Hye Kyung Park Hwan Tae |
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Institution: | Department of Physiology and Medical Science Research Institute, College of Medicine, Dong-A University, 3-1, Dongdaesin-Dong, Seo-Gu, Busan 602-714, South Korea. |
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Abstract: | Tumor endothelial marker 7 (TEM7) is a novel transmembrane protein that is highly expressed in the tumor endothelium. However, the ligands and functions of TEM7 are unknown at present. Using a recombinant ectodomain of TEM7 as a probe, we could identify the saturable interaction between nidogen and TEM7 in vitro. A cell overlay binding assay showed that recombinant nidogen interact with full length TEM7 on cell surface. Finally, nidogen/TEM7 interaction enhanced cell spreading in TEM7 transfected 293T cells. This novel nidogen/TEM7 interaction may provide an important molecular tool with potential therapeutic applications in the anti-angiogenic therapies. |
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Keywords: | TEM7 tumor endothelial marker 7 ECM extracellular matrix |
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