Molecular identification of four Panonychus species (Acari: Tetranychidae) in Korea,including new records of P. caglei and P. mori |
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Authors: | Tin Moe Khaing Jae‐Kyoung Shim Kyeong‐Yeoll Lee |
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Institution: | 1. School of Applied Biosciences, Kyungpook National University, Daegu, Republic of Korea;2. Institute of Plant Medicine, Kyungpook National University, Daegu, Republic of Korea;3. Sustainable Agriculture Research Center, Kyungpook National University, Gunwi, Republic of Korea |
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Abstract: | Four species of the genus Panonychus are identified in Korea including two previously known species P. citri (McGregor) and P. ulmi (Koch) and two newly identified species P. mori Yokoyama and P. caglei Mellot. Morphological diagnostics were observed among the four species in the color of dorsal tubercles and in the shape of male aedeagus. The dorsal tubercles of P. citri, P. ulmi, P. mori and P. caglei are red, white, whitish red and light red, respectively. The aedeagi of male adults are diagnostic in sigmoid‐shaped at the distal end and its shape and length was different among four species. Molecular comparison of the internal transcribed spacer 2 (ITS2) sequence of nuclear DNA and cytochrome‐c oxidase subunit I (COI) nucleotide sequence of mitochondrial DNA among the four species showed divergences 8–12% and 9–12%, respectively. Molecular analysis of the ITS2 and COI sequences revealed their divergences were slightly different among four species. In addition, species‐specific primer sets were designed at the base on ITS2 sequences to precisely diagnose these four species at the molecular level. |
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Keywords: | COI ITS2 molecular diagnosis spider mites |
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