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Purification of glycyrrhizin from Glycyrrhiza uralensis Fisch with ethanol/phosphate aqueous two phase system
Authors:Tan Tianwei  Huo Qing  Ling Qiang
Affiliation:(1) Department of Biochemical Engineering, Beijing University of Chemical Technology, Beijing, 100029, P.R. China
Abstract:Phase diagrams of alcohol (ethanol or 2-propanol)/salt (phosphate or sulfate) aqueous two-phase systems were made. The system consisting of 60% (v/v) ethanol and 15% (w/v) phosphate was then used to separate glycyrrhizin from an extract of Glycyrrhiza uralensis Fisch and gave a 92% recovery of glycyrrhizin with 2.6-fold purification.
Keywords:aqueous two phase system  ethanol/phosphate system  Glycyrrhiza uralensis Fisch  glycyrrhizin  separation
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