Comparison of growth and cell proliferation kinetics during mouse molar odontogenesis in vivo and in vitro |
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Authors: | N. Ahmad J. V. Ruch |
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Affiliation: | Institut de Biologie Médicale, CNRS LP 6520, UniversitéLouis Pasteur, Strasbourg, France |
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Abstract: | Abstract. The growth of embryonic first lower mouse molars in vitro was slow and reduced in comparison with in vivo development: the volume of teeth removed on day 15 and 16 of gestation and cultured for 6 days did not exceed the volume reached at day 18 in vivo . The volume of teeth removed on day 17 and 18 and cultured for 6 days either remained constant or decreased. The appearance of post-mitotic odontoblasts and ameloblasts was delayed in vitro . This behaviour might be correlated with a lengthening of the cell cycle ( T c). In vitro , the average durations of T c (established by the percentage labelled mitoses technique) were 17.4–20.2 hr and 19.1–19.4 hr for pre-odontoblasts and pre-ameloblasts respectively. In vivo , the corresponding T c values were 13.9 hr and 13.5 hr. The coordination of mitotic activities of pre-odontoblasts and pre-ameloblasts existing in vivo was maintained in vitro , and therefore seemed to require intra-dental control mechanisms. Non-specific extra-dental serum factors may affect the duration of T c. |
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