Plant-dependent active biological containment system for recombinant rhizobacteria |
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| Authors: | van Dillewijn Pieter Vílchez Susana Paz José A Ramos Juan L |
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| Affiliation: | Department of Biochemistry and Molecular and Cellular Biology of Plants, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Cientificas, Apdo Correos 419, C/Profesor Albareda 1, E-18008 Granada, Spain. |
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| Abstract: | This work describes the construction of the rhizobacterium Pseudomonas putida strain CS-4, which contains an active biological containment (ABC) system that ensures bacterial suicide in the absence of proline. Maize plants exudate proline, which allows CS-4 to colonize the rhizosphere at a similar level to that of the wild-type strain. However, when the plants are removed, the CS-4 population decreased in bulk soil at a higher rate than the wild-type strain. |
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