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The ultrastructure of polygonal networks in chick embryonic cells in vitro
Authors:G W Ireland  E J Sanders  F C Voon  J Wakely
Affiliation:1. Department of Hematologic Oncology and Blood Disorders, Taussig Cancer Institute, Cleveland Clinic, Cleveland, OH;2. Case Comprehensive Cancer Center, Cleveland, OH;3. Department of Translational Hematology and Oncology Research, Taussig Cancer Institute, Cleveland Clinic, Cleveland, OH;4. Interdisciplinary Nanotechnology Centre, Aligarh Muslim University, Aligarh, India;5. Taussig Cancer Institute, Department of Quantitative Health Sciences, Cleveland Clinic, Cleveland, OH;6. Department of Hematology and Oncology, Seidman Cancer Center, University Hospitals, Cleveland, OH;7. University of Michigan Comprehensive Cancer Center, Ann Arbor, MI;8. Department of Blood and Marrow Transplant and Cellular Immunotherapy, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL;9. Zymo Research, Irvine CA;10. Lerner Research Institute, Genomic Core Facility, Cleveland Clinic, Cleveland, OH;11. Department of Epidemiology, University of Arkansas for Medical Sciences, Little Rock, AR;12. Rockefeller Cancer Institute, University of Arkansas for Medical Sciences, Little Rock, AR;13. Department of Malignant Hematology, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL;14. Morsani College of Medicine, University of South Florida, Tampa, FL;15. Department of Clinical Pathology, Menoufia University, Shebin-Elkom, Egypt
Abstract:Polygonal networks in cultured chick endoderm cells are ordered arrays of actin microfilaments situated just beneath the dorsal cell surface. Each strut is formed from a bundle of microfilaments and 5-7 bundles intersect at each node. Dense bodies are seen in nodes and some struts. At its periphery the network is attached to the substrate at the termini of long radial struts. Most of the network is resistant to detergent extraction. Sliding microfilaments can explain the observed behaviour of networks in live cells.
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