Steric analysis of glycerophospholipids by circular dichroism. Stereospecifity of phospholipase D catalyzed transesterification. |
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Authors: | S G Batrakov A G Panosyan G A Kogan L D Bergelson |
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Institution: | Shemyakin Institute of Bioorganic Chemistry USSR Academy of Science Moscow, USSR |
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Abstract: | The circular dichroism spectra of natural glycerophospholipids and synthetic 1--phosphatidic acid were recorded. 3--phosphatidic acid derivatives were found to show a positive Cotton effect, while 1--phosphatidic acid revealed a negative Cotton effect. The results are interpreted in terms of the carboxyl sector rule. By this method phospholipase D was shown to produce stereospecifically 3--phosphatidyl-1--glycerol when incubated with egg yolk lecithin and exess of glycerol. |
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