Siderophore-mediated iron acquisition in the entomopathogenic bacterium Pseudomonas entomophila L48 and its close relative Pseudomonas putida KT2440 |
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Authors: | Sandra Matthijs Georges Laus Jean-Marie Meyer Kourosch Abbaspour-Tehrani Mathias Schäfer Herbert Budzikiewicz Pierre Cornelis |
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Institution: | 1. Laboratory of Microbial Interactions, Department of Molecular and Cellular Interactions, Flanders Interuniversity Institute for Biotechnology, Vrije Universiteit Brussel, Building E, Pleinlaan 2, 1050, Brussels, Belgium 2. Laboratory of Organic Chemistry, Vrije Universiteit Brussel, Pleinlaan 2, 1050, Brussels, Belgium 3. Département Génétique Moléculaire, Génomique, Microbiologie, UMR, 7156, CNRS/Université Louis-Pasteur, 28 rue Goethe, 67000, Strasbourg, France 4. Institut für Organische Chemie, Universit?t zu K?ln, Greinstrasse 4, 50939, K?ln, Germany
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Abstract: | Pseudomonas entomophila L48 is a recently identified entomopathogenic bacterium which, upon ingestion, kills Drosophila melanogaster, and is closely related to P. putida. The complete genome of this species has been sequenced and therefore a genomic, genetic and structural analysis of the siderophore-mediated
iron acquisition was undertaken. P. entomophila produces two siderophores, a structurally new and unique pyoverdine and the secondary siderophore pseudomonine, already described
in P. fluorescens species. Structural analysis of the pyoverdine produced by the closely related P. putida KT2440 showed that this strain produces an already characterised pyoverdine, but different from P. entomophila, and no evidence was found for the production of a second siderophore. Growth stimulation assays with heterologous pyoverdines
demonstrated that P. entomophila is able to utilize a large variety of structurally distinct pyoverdines produced by other Pseudomonas species. In contrast, P. putida KT2440 is able to utilize only its own pyoverdine and the pyoverdine produced by P. syringae LMG 1247. Our data suggest that although closely related, P. entomophila is a more efficient competitor for iron than P. putida. |
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