Characterization of a new spontaneously developed murine mammary adenocarcinoma in syngeneic BALB/c hosts |
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Authors: | Tsu-Yi Chao T Ming Chu |
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Institution: | (1) Department of Diagnostic Immunology Research and Biochemistry, Roswell Park Memorial Institue, 666 Elm Street, 14263 Buffalo, New York |
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Abstract: | Summary A mouse mammary tumor cell line, desingated JC, has been established from a spontaneously developed primary adenocarcinoma
of an aged virgin female BALB/c mouse. Isoenzyme analyses including glucose-6-phosphate dehydrogenase, lactate dehydrogenase,
and peptidase proved that this cell line is of murine origin and devoid of contamination from other species. Karyotyping revealed
that the number of chromosome ranged from 26 to 100, with a modal number of 40. Electron microscopic examination detected
the presence of tonofilament and desmosomes confirming its epithelial nature. In addition, no type B or C virus particle was
detected, although intracysternal A particle was observed occasionally. Tumorigenicity in immunocompetent syngeneic hosts
was easily established by s.c., i.p., and i.v. injection of viable JC tumor cells. A very weak immunogenicity of the JC tumor
was demonstrated through its immunization-challenging on syngeneic immunocompetent hosts. Although no rejection of JC tumor
was noted, a significant prolongation for the incubation period before an obvious and palpable tumor growth was detected between
the experimental and the control animals. Development of a concomitant immunity was also detected. The JC tumor represents
a valuable murine mammary tumor model which is different from other available models because of its unique origin, absence
of virus particles, very weak immunogenicity, and high tumorigenicity in syngeneic hosts. The cell line has been maintained
for more than 5 yr and has been used for experimental immunotherapy in our laboratory.
This work was supported by a research grant IM-416, awarded by the American Cancer Society, Atlanta, Georgia. |
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Keywords: | murine mammary tumor BALB/c mouse immunogenicity |
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