Phospholipid Methylation Increases [3H]Diazepam and [3H]GABA Binding in Membrane Preparations of Rat Cerebellum |
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Authors: | B Di Perri G Calderini A Battistella R Raciti G Toffano |
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Institution: | Department of Biochemistry, Fidia Research Laboratories, Abano Terme;Institute of General Physiology, University of Messina, Messina, Italy |
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Abstract: | The effect of phospholipid methylation on both 3H]diazepam and 3H]GABA ( 3H]gamma-aminobutyric acid) binding to crude synaptic plasma membrane from rat cerebellum has been studied. S-Adenosylmethionine (SAM) stimulates 3H]methyl group incorporation into membrane phospholipids and enhances 3H]diazepam binding by increasing the apparent Bmax. Conversely, inhibition of 3H]methyl group transfer from 3H]SAM to phospholipids by preincubation with SAM at 0 degrees C or with SAH abolishes the increase of binding. After preincubation with SAM, analysis of the GABA binding reveals the presence of binding sites with high affinity, a property absent in control membranes preincubated without SAM. Among the neurotransmitter bindings tested, only those of GABA and benzodiazepine in the cerebellum and beta-adrenergic ligands in the cerebral cortex are enhanced upon stimulation of phospholipid methyltransferase activity. 3H]Dihydromorphine, 3H]dihydro-alpha-ergokryptine and 3H]spiroperidol bindings are not affected by SAM. The present data suggest an involvement of phospholipid methylation in regulation of both 3H]GABA and 3H]-diazepam binding. |
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Keywords: | Diazepam binding GABA binding Enhancement Phospholipid methylation Cerebellar membranes |
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