| 一株菲降解细菌的分离鉴定及其特性 |
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| 引用本文： | 祝儒刚,钟鸣,周启星,刘海宁,李玉双.一株菲降解细菌的分离鉴定及其特性[J].应用生态学报,2006,17(11):2117-2120. |
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| 作者姓名： | 祝儒刚 钟鸣 周启星 刘海宁 李玉双 |
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| 作者单位： | 1.沈阳农业大学辽宁省农业生物技术重点实验室， 沈阳 110161；;2.中国科学院沈阳应用生态研究所陆地生态过程重点实验室， 沈阳 110016 |
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| 基金项目： | 国家高技术研究发展计划(863计划);中国科学院重点实验室基金 |
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| 摘 要： | 通过选择性富集培养，从沈抚灌区石油污染土壤中分离到1株菲降解细菌.试验证明该菌株能以菲为唯一碳源和能源生长.经形态学、生理生化鉴定和16S rRNA基因序列比对分析，确定该菌株属于不动杆菌属，命名为Acinetobacter sp. L2. 系统发育进化分析发现，L2菌株与Acinetobacter sp. DG880［AY258108］亲源关系最近.L2菌株培养7 d后对菲的降解率达96.3%.邻苯二酚2,3-双加氧酶活力测定表明,L2菌株可能含有菲降解基因.
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| 关 键 词： | 河流有机碳 陆地生态系统 全球碳循环 流域侵蚀 14C 人类活动 |
| 文章编号： | 1001-9332(2006)11-2117-04 |
| 收稿时间： | 2005-11-03 |
| 修稿时间： | 2006-08-24 |
Isolation and identification of a phenanthrene-degrading bacterial strain |
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| ZHU Rugang,ZHONG Ming,ZHOU Qixing,LIU Haining,LI Yushuang.Isolation and identification of a phenanthrene-degrading bacterial strain[J].Chinese Journal of Applied Ecology,2006,17(11):2117-2120. |
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| Authors: | ZHU Rugang ZHONG Ming ZHOU Qixing LIU Haining LI Yushuang |
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| Institution: | 1.Key Laboratory of Agricultural Biotechnology of Liaoning Province, Shenyang Agricultural ;University, Shenyang 110161, China;2.Key Laboratory of Terrestrial Ecological Process, Institute of Applied Ecology, Chinese ;Academy of Sciences, Shenyang 110016, China |
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| Abstract: | Through selective enriched culture, a phenanthrene-degrading bacterial strain was isolated from the oil-contaminated soil in Shenfu irrigation area of Shenyang, Northeast China. The morphological and physiological-biochemical identification, 16S rDNA sequence analysis, and phylogenetic study showed that this strain was belonged to genus Acinetobacter and named as Acinetobacter sp, L2, and closest to Acinetobacter sp. DG880 AY258108 ]. It could use phenanthrene as the sole carbon source, Mter 7 days culture, the degradation rate of phenanthrene was 96. 3%. According to the activity of catechol 2,3-dioxygenase, the strain probably had phenanthrene-degrading genes. |
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| Keywords: | 16SrRNA Acinetobacter |
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