Nonhistone nuclear proteins specific to certain mouse embryonal carcinoma clones having an inactive X chromosome |
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Authors: | Kenji Abe Nobuo Takagi Motomichi Sasaki |
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Institution: | Chromosome Research Unit, Faculty of Science, Hokkaido University, Sapporo, Japan. |
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Abstract: | By means of one-dimensional polyacrylamide slab gel electrophoresis nonhistone nuclear proteins were compared in murine embryonal carcinoma cell clones with two X chromosomes; both are active in some clones and one of them is inactive in others and in a population of cells having only one X chromosome. Under our experimental conditions, we succeeded in finding two extra bands at approximately 46,000 Da in cells having an inactive X chromosome. Furthermore, a band at approximately 71,000 Da was significantly heavier in cells having an inactive X chromosome than in those having two active X or those having only one X chromosome. |
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