Isolation and characterization of a novel thermophilic Bacillus strain degrading long-chain n-alkanes |
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| Authors: | Lei Wang Yun Tang Shuo Wang Ru-Lin Liu Mu-Zhi Liu Yan Zhang Feng-Lai Liang Lu Feng |
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| Affiliation: | (1) TEDA School of Biological Sciences and Biotechnology, Nankai University, 23# Hong Da Street, TEDA, 300457 Tianjin, People’s Republic of China;(2) Tianjin Key Centre for Functional Genomics and Biochip Research, TEDA College, Nankai University, 23# Hong Da Street, TEDA, 300457 Tianjin, People’s Republic of China;(3) Tianjin Key Laboratory of Food Nutrition and Safety, Faculty of Food Engineering and Biotechnology, Tianjin University of Science and Technology, 300222 Tianjin, People’s Republic of China;(4) College of Life Sciences, Nankai University, 300071 Tianjin, People’s Republic of China |
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| Abstract: | A thermophilic Bacillus strain NG80-2 growing within the temperature range of 45–73°C (optimum at 65°C) was isolated from a deep subterranean oil-reservoir in northern China. The strain was able to utilize crude oil and liquid paraffin as the sole carbon sources for growth, and the growth with crude oil was accompanied by the production of an unknown emulsifying agent. Further examination showed that NG80-2 degraded and utilized only long-chain (C15–C36) n-alkanes, but not short-chain (C8–C14) n-alkanes and those longer than C40. Based on phenotypic and phylogenic analyses, NG80-2 was identified as Geobacillus thermodenitrificans. The strain NG80-2 may be potentially used for oily-waste treatment at elevated temperature, a condition which greatly accelerates the biodegradation rate, and for microbial enhancing oil recovery process.Lei Wang, Yun Tang and Shuo Wang contributed equally to this study. |
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| Keywords: | Long-chain n-alkanes Degradation Geobacillus thermodenitrificans Thermophile Characterization |
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