Suitability of cryoprotectants and impregnation protocols for embryos of Japanese whiting Sillago japonica |
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| Authors: | Rahman Sk Mustafizur Majhi Sullip Kumar Suzuki Toru Matsukawa Shingo Strüssmann Carlos Augusto Takai Rikuo |
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| Affiliation: | Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato-Ku, Tokyo 108-8477, Japan. |
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| Abstract: | The purpose of this study was to examine the suitability of cryoprotectant agent (CPA) impregnation protocols for the embryos of Japanese whiting (Sillago japonica), a small-sized, easy-to-rear, and prolific marine fish which may constitute a suitable experimental material for the development of cryopreservation methods for fish embryos. Our immediate goals were to assess the toxicity and permeability of various CPAs to whiting embryos of different developmental stages. Exposure of gastrula, somites, tail elongation, and pre-hatching embryos to 10%, 15%, and 20% solutions of propylene glycol (PG), methanol (MeOH), dimethyl sulfoxide (Me2SO), dimethylformamide (DFA), ethylene glycol (EG), and glycerol (Gly) in artificial sea water (ASW; 33 psu) for 20 min revealed that CPA toxicity for whiting embryos increased in the order of PG
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