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Application of phage-displayed single chain antibodies in western blot
Authors:Ding Yan-Li  Liu Mei-Yun  Han Wei  Yang Sheng-Li  Liu Hui  Gong Yi
Institution:School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, China.
Abstract:A phage display single chain fragment variable library constructed on pIII protein of M13 filamentous phage was screened using B-lymphocyte stimulator and FP248 as selective molecules. After four rounds of panning, there was a remarkable enrichment in the titer of bound phages. Twenty phage clones were selected from the last round and screened by means of phage-ELISA. With the antibody phages as primary antibodies in Western blot, we developed a method for detecting the specific antigen. The dilutions of antibody phages depend on the affinity between antibody-displayed phage particles and antigens.
Keywords:phage-display  antibody  Western blot
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