Application of phage-displayed single chain antibodies in western blot |
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Authors: | Ding Yan-Li Liu Mei-Yun Han Wei Yang Sheng-Li Liu Hui Gong Yi |
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Institution: | School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, China. |
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Abstract: | A phage display single chain fragment variable library constructed on pIII protein of M13 filamentous phage was screened using B-lymphocyte stimulator and FP248 as selective molecules. After four rounds of panning, there was a remarkable enrichment in the titer of bound phages. Twenty phage clones were selected from the last round and screened by means of phage-ELISA. With the antibody phages as primary antibodies in Western blot, we developed a method for detecting the specific antigen. The dilutions of antibody phages depend on the affinity between antibody-displayed phage particles and antigens. |
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Keywords: | phage-display antibody Western blot |
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