In vitro spleen cell responsiveness to various analogs of MDP (N-acetylmuramyl-L-alanyl-D-isoglutamine), a synthetic immunoadjuvant, in MDP high-responder mice |
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Authors: | C Damais M Parant L Chedid P Lefrancier J Choay |
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Institution: | 1. Immunothérapie Expérimentale, Institut Pasteur, and Groupe de Recherche No. 31 du Centre National de la Recherche Scientifique Paris, France;2. Institut Choay, Paris, France |
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Abstract: | N-Acetylmuramyl-l-alanyl-d-isoglutamine (MDP), a synthetic immunoadjuvant, was incubated with spleen cells of DBA/2 or Balb/c mice and optimal responses were obtained after 4 or 5 days of culture in a serum-free medium supplemented with 2-mercaptoethanol. In contrast, lymphocytes of (C57B1/6 × AKR)F1 hybrids responded weakly under the same conditions. The results reported here show that like in the case of DBA/2 and Balb/c strains, spleen cells of Swiss mice and of inbred AKR and CBA mice could be stimulated in vitro whereas C57B1/6 and LPS-refractory C3H/He mice did not respond. Fourteen synthetic MDP analogs (eight known to be adjuvant active and six devoid of activity) were tested in DBA/2 high-responder mice. A good correlation was observed between in vitro stimulation and the presence or absence of adjuvant activity in vivo of these compounds. |
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Keywords: | Address reprint requests to Dr Louis Chedid Immunothérapie Expérimentale Institut Pasteur 28 rue du Dr Roux 75724 Paris Cedex 15 France |
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