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Studies on the Growth in Culture of Plant Cells: II. CHANGES IN RESPIRATION RATE AND NITROGEN CONTENT ASSOCIATED WITH THE GROWTH OF ACER PSEUDOPLATANUS L. CELLS IN SUSPENSION CULTURE
Authors:GIVAN  CURTIS V; COLLIN  H A
Abstract:Changes in nitrogen content and in respiration rate have beeninvestigated in cell suspension cultures of Acer pseudoplatanus.Nitrogen content and rate of oxygen uptake rise sharply earlyin the period of culture, during which there is no significantincrease in dry weight and only a small increase in cell number.During the subsequent period of rapid cell division there isa decline in both respiration rate and nitrogen content permg dry weight or per cell. Pronounced rises in respiration rateand cell nitrogen therefore occur prior to the period of rapidcell division. The strong correlation between nitrogen contentand oxygen consumption suggests that the respiration rate ismuch more closely related to changes in protein content thanto changes in cell number, dry weight, or packed-cell volume.
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