Three-day continuous drainage of pancreatic juice in the cortisone-treated conscious rat: Analysis of enzyme activities and ultrastructural changes |
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Authors: | Hermann Gartemann Martin Zeitz Carsten Emde Manfred Stelzer Prof Dr med Ernst Otto Riecken |
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Institution: | (1) Department of Gastroenterology, Klinikum Steglitz, Free University of Berlin, Berlin, Federal Republic of Germany;(2) Klinikum Steglitz der FU Berlin, Medizinische Klinik und Poliklinik mit Schwerpunkt Gastroenterologie, Hindenburgdamm 30, 1000 Berlin 45, Federal Republic of Germany |
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Abstract: | Summary We have investigated the short-term effects of hydrocortisone (60 mg/kg per day) and placebo on basal and stimulated pancreatic secretion in the conscious rat. Volume and enzyme secretion were determined; fine structural changes were examined simultaneously.The pancreatic and bile ducts were cannulated separately; pancreatic juice was drained via an isolated fistula, and bile was recirculated into the duodenum. The application of hydrocortisone led to an almost complete inhibition of the secretory response of the exocrine pancreas when stimulated with 0.25 U secretin in combination with 5 × 10-8 g caerulein per h. It strongly affected the secretion rates of volume, protein, lipase, chymotrypsin, trypsin and carboxypeptidase, whereas the secretion rate of alpha-amylase continued to show a slight increase after stimulation.After stimulation with secretin and caerulein, the hydrocortisone-treated animals showed a higher density of zymogen granules in the acinar cell and an increase in the number of autophagic vacuoles in comparison to the equally stimulated placebo-treated rats.It is concluded that the short-term inhibition of pancreatic secretion by hydrocortisone occurs largely as a result of an inhibition of cellular enzyme discharge.Supported by the Deutsche Forschungsgemeinschaft, Ga 279 |
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Keywords: | Exocrine pancreas Rat Cortisone Ultrastructure Enzyme secretion |
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