Antioxidant activity of the halophyte Limonium tetragonum and its major active components |
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Authors: | Jung Im Lee Chang-Suk Kong Myoung Eun Jung Joo Wan Hong Sun Young Lim Youngwan Seo |
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Institution: | 1. Division of Marine Environment and Bioscience, Korea Maritime University, Busan, 606-791, Korea 2. Department of Food and Nutrition, Silla University, Busan, 617-736, Korea
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Abstract: | In this study, the antioxidant potentials of crude extracts and solvent-partitioned fractions of Limonium tetragonum were assessed by measuring their ability to scavenge intracellular reactive oxygen species (ROS) generated in HT-1080 cells. Following activity-oriented separation, four flavonol glycosides were isolated as active principles and their chemical structures were determined by 2 D NMR and by comparison with reported spectral data. The isolated compounds (1?C4) were evaluated for their antioxidant capacity using three different activity tests; degree of occurrence of intracellular ROS, lipid peroxidation in HT-1080 cells and the extent of oxidative damage of genomic DNA purified from HT-1080 cells. All compounds exhibited significantly inhibited the generation of intracellular ROS and lipid peroxidation in HT-1080 cells, and significantly inhibited DNA oxidation. In addition, direct free radical scavenging effects of these compounds were investigated using the electron spin resonance (ESR) spin-trap technique. |
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