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Enhanced saccharification of rice straw using hypochlorite-hydrogen peroxide |
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| Authors: | Hee-Kyoung Kang Nahyun M Kim Ghahyun J Kim Eun-Seong Seo Hwa-Ja Ryu Sang-Il Yun Hyun-Chul Choi Donal F Day Jongho Kim Dong-Lyun Cho Doman Kim |
| Institution: | 1. The Research Institute for Catalysis, Chonnam National University, Gwang-ju, 500-757, Korea 2. Department of Environmental Studies, University of Southern California, Los Angeles, CA, 90089, USA 3. Department of Biology, University of California San Diego, San Diego, CA, 92093, USA 4. School of Biological Sciences and Technology, Chonnam National University, Gwang-ju, 500-757, Korea 5. Interdisciplinary Program of Graduate School for Bioenergy and Biomaterials, Chonnam National University, Gwang-ju, 500-757, Korea 6. Audubon Sugar Institute, Louisiana State University, Pennington, LA, 70776, USA 7. School of Applied Chemical Engineering, Chonnam National University, Gwang-ju, 500-757, Korea |
| Abstract: | Rice straw is a lignocellulosic biomass, and has been recognized as a renewable organic substance and alternative energy source. In this study, rice straw was pretreated with hypochlorite-hydrogen peroxide (Ox-B) solution. The optimal pretreatment conditions were determined via response surface methodology, and the pretreated rice straw was hydrolyzed with exo-glucanase, endoglucanase, hemicellulase, and β-glucosidase Accellerase 1000? (endo-glucanase equivalent activity of 1,250 carboxy methyl cellulose (CMC) U/g of rice straw pretreated for 24 h). The optimal conditions were as follows: 60 min pretreatment using Ox-B solution containing 0.6% hypochlorite and 25% hydrogen peroxide for 1 g of rice straw in a total reaction volume of 240 mL. Under these conditions, 406.8 mg of d-glucose and 224.0 mg of d-xylose were obtained from 1 g of rice straw. The fermentation of enzymatic hydrolysates containing 8.14 g/L d-glucose and 4.49 g/L d-xylose with Pichia stipitis generated 3.65 g/L of ethanol with a corresponding yield of 0.37 g/g. The maximum possible ethanol conversion rate is 72.54%. |
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