Comparison of Changes in GAD65 and GAD67 Immunoreactivity and Levels in the Gerbil Main Olfactory Bulb Induced by Transient Ischemia |
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Authors: | Ki-Yeon Yoo In Koo Hwang Jung Hoon Choi Je Kyung Seong Yeo Sung Yoon In Se Lee Moo-Ho Won |
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Institution: | (1) Department of Anatomy and Neurobiology, College of Medicine, Hallym University, Chuncheon, 200-702, South Korea;(2) Department of Anatomy and Cell Biology, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Seoul, 151-742, South Korea |
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Abstract: | In the present study, we investigated changes in glutamate decarboxylase 65 (GAD65) and GAD67 immunoreactivity and protein
levels in the main olfactory bulb (MOB) after 5 min of transient forebrain ischemia in gerbils. GAD65 immunoreactivity in
the sham-operated group was shown in neurons and neuropil except for the somata of granule cells. GAD65 immunoreactivity was
increased in neurons in the external plexiform layer 60 days after ischemia, and in mitral cells 30 and 60 days after ischemia.
GAD67 immunoreactivity in the sham-operated group was shown in periglomerular cells, neuron in the external plexiform layer
and granule cells with neuropil. GAD67 immunoreactivity in periglomerular cells was increased 10, 45 and 60 days after ischemia.
GAD67 immunoreactivity in neurons in the external plexiform layer was increased 10 and 15 days after ischemia. Mitral cells
showed strong GAD67 immunoreactivity 10 days after ischemia. However, GAD67 immunoreactivity in the granule cells was not
changed with time after ischemia. In Western blot analysis for GAD65 and GAD67 protein levels in the ischemic gerbil MOB,
GAD65 level was not changed after ischemia; GAD67 level was increased 10 days after ischemia. These results suggest that transient
ischemia causes changes in GAD65 and GAD67 immunoreactivity in the gerbil MOB, and this change may induce a malfunction in
olfaction after an ischemic insult.
Ki-Yeon Yoo and In Koo Hwang equally contributed to this article. |
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Keywords: | Main olfactory bulb Transient ischemia GABA GAD65 GAD67 Gerbil |
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