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Histochemical and ultrastructural identification of neurotensin cells in the dog ileum
Authors:B Frigerio  M Ravazola  S Ito  R Buffa  C Capella  E Solcia  L Orci
Institution:(1) Histopathology, Histochemistry and Ultrastructure Centre, University of Pavia at Varese, Viale L. Borri, 57, I-21100 Varese, Italy;(2) Institute of Histology and Embryology, University of Geneva Medical School, Geneva, Switzerland
Abstract:Summary In the dog ileum, neurotensin cells stained with immunofluorescence or immunoperoxidase proved distinct from argentaffin (EC) cells, glucagon immunoreactive (GLI) cells and pancreatic peptide immunoreactive (PP) cells. Neurotensin cells showed various degrees of reactivity with Grimelius'silver. With electron microscopy, besides EC cells, large granule cells with a thin peripheral rim of Grimelius-reactivity (L cells) and large granule cells with variable Grimelius-reactivity of the core (N cells) were found. On distributive grounds, L cells were identified with GLI cells and N cells were interpreted as neurotensin cells.
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