Critical stresses for cancer cell detachment in microchannels |
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Authors: | Cécile Couzon Alain Duperray Claude Verdier |
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Institution: | 1.Laboratoire de Spectrométrie Physique (UMR 5588),CNRS, Université Grenoble I,Saint-Martin d’Hères cedex,France;2.INSERM U823,La Tronche cedex,France;3.Faculté de Médecine, Institut d’oncologie/développement Albert Bonniot et Institut Fran?ais du Sang (UMR S823),Université Joseph Fourier-Grenoble I,Grenoble,France |
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Abstract: | We present experiments involving cancer cells adhering to microchannels, subjected to increasing shear stresses (0.1–30 Pa).
Morphological studies were carried out at different shear stresses. Cells exhibit spreading patterns similar to those observed
under static conditions, as long as the shear stress is not too high. At critical wall shear stresses (around 2−5 Pa), cell-substrate
contact area decreases until detachment at the larger stresses. Critical shear stresses are found to be lower for higher confinements
(i.e. smaller cell height to channel height ratio). Fluorescent techniques were used to locate focal adhesions (typically
1 μm2 in size) under various shearing conditions, showing that cells increase the number of focal contacts in the region facing
the flow. To analyze such data, we propose a model to determine the critical stress, resulting from the competition between
hydrodynamic forces and the adhesive cell resistance. With this model, typical adhesive stresses exerted at each focal contact
can be determined and are in agreement with previous works. |
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