DNA,RNA, protein and DNases in developing rat cerebellum: Effects of early postnatal nutritional deprivation

The effect of early postnatal undernutrition and subsequent rehabilitation on wet weight, DNA, RNA, protein and the activities of acid and alkaline DNases in the cerebellar region of rat brain was studied. The cerebellar region was found to be affected significantly during early undernutrition. Further, earlier the initiation of nutritional rehabilitation the better was the recovery and in some cases timely nutritional rehabilitation resulted in better than normal biochemical composition of the brain. The specific activities of acid and alkaline DNases were not affected by early undernutrition. However, the total activities of these enzymes were significantly low in undernourished rats (R1₁₅ and R₂₁) Rehabilitation of these deprived groups upto 150 days resulted in higher amounts of these enzymes as compared to those of age-matched controls. It is concluded that the two DNases, are synthesized in a preferential manner during rehabilitation, It is further concluded that cerebellar region, in terms of development schedule and response to imposed calorie restriction, is intermediary between grey and white matter regions.