Isolation and characterization of cDNAs encoding oat 12S globulin mRNAs

Summary A cDNA library was made from poly(A⁺) RNA isolated from developing oat seeds, and oat globulin cDNA clones were identified by hybridization with synthetic oligonucleotides. Globulin clones were characterized by restriction enzyme mapping and cross-hybridization analysis. Based on these comparisons, four classes of globulin clones were distinguished. These clones hybridized to multiple DNA fragments in restriction enzyme digests of oat genomic DNA, indicating that the genes exist in a multigene family. The nucleotide sequence of one of the globulin cDNA clones was determined. The amino acid sequence derived from the DNA sequence verified its identity as an oat globulin and confirmed that the protein is synthesized as a precursor similar to legume 11S storage globulins. The basic polypeptide encoded at the 3 end of the mRNA was found to be homologous to the basic polypeptides of other 11S seed globulins.Abbreviations ds double stranded - kb kilobase Author to whom correspondence should be addressed. Journal paper number 10460 of the Purdue Agricultural Experimental Station.