Effects of activation of skin ion channels TRPM8, TRPV1, and TRPA1 on the immune response. Comparison with effects of cold and heat exposure |
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| Institution: | 1. INSERM U894 – CPN, Université Pierre et Marie Curie – Paris 6, Faculté de Médecine Pierre et Marie Curie, Site Pitié-Salpêtrière, UMR S677, 91, Boulevard de l''Hôpital, Paris F-75013, France;2. Département de Pharmacologie, Université Pierre et Marie Curie – Paris 6, Faculté de Médecine Pierre et Marie Curie, Site Pitié-Salpêtrière, 91, Boulevard de l''Hôpital, Paris F-75013, France;1. Department of Molecular and Cellular Neurobiology, Gunma University Graduate School of Medicine, Maebashi 371-8511, Japan;2. Division of Cell Signaling, National Institute for Physiological Sciences, Okazaki 444-8787, Japan;3. Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, Okazaki 444-8787, Japan;4. Department of Physiological Sciences, The Graduate University for Advanced Studies, Okazaki 444-8585, Japan;1. Institute of Physiology and Fundamental Medicine, Russian Academy of Sciences, Timakov Str. 4, Novosibirsk 630117, Russia;2. Novosibirsk State University, Pirogov Str. 2, Novosibirsk 630090, Russia;1. Center for Biomedical Research on Pain (CBRP), College of Medicine, Xi''an Jiaotong University, Xi''an 710061, PR China;2. Department of Rehabilitation Medicine, Tongji Hospital Affiliated to Shanghai Tongji University, Shanghai 200065, PR China;3. MOE Key Laboratory of Thermo-Fluid Science and Engineering, School of Energy and Power Engineering, Xi’an JiaoTong University, Xi’an 710049, PR China;4. Institute of Biomedicine/Physiology, University of Helsinki, POB 63, Helsinki 00014, Finland |
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| Abstract: | The effects of pharmacological stimulation of skin ion channels TRPA1, TRPM8, TRPV1 on the immune response are presented. These effects are compared with the effects of different types of temperature exposures - skin cooling, deep cooling, and deep heating. This analysis allows us to clear the differences in the influence on the immune response of thermosensitive ion channels localized in the skin; (2) whether the changes in the immune response under temperature exposures are due to these thermosensitive ion channels. Experiments were performed on Wistar rats. For stimulation of TRPM8 ion channel, an application to the skin of 1% menthol was used, for TRPA1 - 0.04% allylisotiocianate, and for TRPV1 - capsaicin in a concentration of 0.001.The antigen binding in the spleen was two-times stimulated by activation of the cold-sensitive ion channel TRPM8 and much weaker by activation of warm-sensitive TRPV1 (by 15%), and another cold-sensitive ion channel TRPA1 (by 40%). Only the stimulation of TRPA1 significantly (by 140%) increased antibody formation in the spleen, while TRPM8 had practically no effect on this process, and activation of TRPV1 significantly (by 60%) inhibited antibody formation. Stimulation of the TRPM8 ion channel significantly (by 60%) reduced the level of IgG in the blood, which is believed to control of infectious diseases.The obtained results show that pharmacological activation of the skin TRPA1, TRPM8, TRPV1 ion channels can differently affect the immune system. At the epicenter of changes there were the antigen binding and antibody formation in the spleen, as well as the level of IgG in the blood. Exactly stimulation of the TRPM8 ion channel determines the changes in the immune response when only the skin is cooling, while at deep body heating, the changes in the immune response are mostly determined by the activation of the skin TRPV1 ion channel. |
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| Keywords: | TRPM8 TRPA1 TRPV1 Cooling Heating Immune response |
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