LncRNA CALB2 sponges miR-30b-3p to promote odontoblast differentiation of human dental pulp stem cells via up-regulating RUNX2 |
| |
| Institution: | 1. Research Team for Geriatric Pathology, Tokyo Metropolitan Institute of Gerontology, Tokyo 173-0015, Japan;2. Department of Judotherapy, Faculty of Health Sciences, Tokyo Ariake University of Medical and Health Sciences, Tokyo 135-0063, Japan;3. Department of Analytical Chemistry, Yokohama College of Pharmacy, Yokohama 245-0066, Japan;4. Department of Pathology, Tokyo Metropolitan Geriatric Hospital, Tokyo 173-0015, Japan;5. Research Team for Vascular Medicine, Tokyo Metropolitan Institute of Gerontology, Tokyo 173-0015, Japan;6. Department of Reproductive Biology, National Research Institute for Child Health and Development, Tokyo 157-8535, Japan;1. Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan;2. Pathogenetic Veterinary Science, United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu-shi, Gifu 501-1193, Japan;3. Chemicals Evaluation and Research Institute, 1-4-25 Koraku, Bunkyo-ku, Tokyo 112-0004, Japan;1. Department of Cardiovascular Surgery, Henan Provincial People''s Hospital of Henan University, Zhengzhou, Henan, 461464, China;2. Department of Cardiovascular Surgery, Fuwai Central China Cardiovascular Hospital, Zhengzhou, Henan, 461464, China;1. Department of Endodontics, Periodontics and Prosthodontics, School of Dentistry, University of Maryland, Baltimore, Maryland;2. Department of Bioscience Research, College of Dentistry, University of Tennessee Health Science Center, Memphis, Tennessee;3. Department of Endodontics, University of North Carolina, Chapel Hill, North Carolina;1. Guanghua School of Stomatology, Hospital of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, 56 Lingyuanxi Road, Guangzhou, Guangdong, 510055, China;2. Foshan Stomatology Hospital, School of Stomatology and Medicine, Foshan University, No. 5, Hebin Road, Chancheng District, Foshan, Guangdong, 528000, China |
| |
| Abstract: | Illuminating the mechanisms of odontoblast differentiation of human dental pulp stem cells (hDPSCs) is the key to find therapeutic clues to promote odontogenesis. LncRNAs play a regulatory role in odontoblast differentiation. Here, we identified a novel lncRNA, named lncRNA CALB2. It was up-regulated in odontoblast-differentiated hDPSCs and potentially interacted with miR-30b-3p and RUNX2. Via gain- and loss-of-function approaches, we found lncRNA CALB2 significantly promoted the odontoblast differentiation of hDPSCs. Then, dual luciferase reporter assay and RNA immunoprecipitation assay revealed that both lncRNA CALB2 and RUNX2 mRNA could directly bind to miR-30b-3p via the same binding sites. Interestingly, miR-30b-3p in hDPSCs was down-regulated and RUNX2 was up-regulated during odontoblast differentiation. Moreover, lncRNA CALB2 knockdown significantly reduced the protein level of RUNX2, DSPP and DMP-1, while miR-30b-3p inhibitor rescued the reduction. Furthermore, miR-30b-3p exerted an inhibitory effect on odontoblast differentiation, which could be reversed by lncRNA CALB2. Collectively, these findings indicate that the newly identified lncRNA CALB2 acts as a miR-30b-3p sponge to regulate RUNX2 expression, thus promoting the odontoblast differentiation of hDPSCs. LncRNA CALB2/miR-30b-3p/RUNX2 axis could be a novel therapeutic target for accelerating odontogenesis. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
