Functionally associated molecular genetic marker map construction in perennial ryegrass (Lolium perenne L.) |
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| Authors: | M J Faville A C Vecchies M Schreiber M C Drayton L J Hughes E S Jones K M Guthridge K F Smith T Sawbridge G C Spangenberg G T Bryan J W Forster |
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| Institution: | (1) Grasslands Research Centre, AgResearch Ltd., Private Bag 11008, Palmerston North, New Zealand;(2) Primary Industries Research Victoria, Plant Biotechnology Centre, La Trobe University, Bundoora, VIC, 3086, Australia;(3) Molecular Plant Breeding Cooperative Research Centre, Australia;(4) AgResearch Limited, School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand;(5) Primary Industries Research Victoria, Hamilton Centre, Private Bag 105, Hamilton, VIC, 3300, Australia;(6) Present address: School of Biological Sciences and Biotechnology, Division of Science and Engineering, Murdoch University, South Street, Murdoch, WA, 6150, Australia;(7) Present address: Crop Genetics Research and Development, Pioneer Hi-Bred International, 7300 NW 62nd Avenue, Johnston, IA 50131-1004, USA;(8) Present address: Cell Cycle and Development Laboratory, Peter MacCallum Cancer Centre, St. Andrew
tm)s Place, East Melbourne, VIC, 3002, Australia;(9) Present address: Molecular Bioscience Technologies, Department of Primary Industries and Fisheries, Queensland Biosciences Precinct, The University of Queensland, Level 6 North Tower, 306 Carmody Road, St. Lucia, 4072, Australia |
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| Abstract: | A molecular marker-based map of perennial ryegrass (Lolium perenne L.) has been constructed through the use of polymorphisms associated with expressed sequence tags (ESTs). A pair-cross between genotypes from a North African ecotype and the cultivar Aurora was used to generate a two-way pseudo-testcross population. A selection of 157 cDNAs assigned to eight different functional categories associated with agronomically important biological processes was used to detect polymorphic EST RFLP loci in the F1(NA6 í AU6) population. A comprehensive set of ESTSSR markers was developed from the analysis of 14,767 unigenes, with 310 primer pairs showing efficient amplification and detecting 113 polymorphic loci. Two parental genetic maps were produced: the NA6 genetic map contains 88 ESTRFLP and 71 ESTSSR loci with a total map length of 963 cM, while the AU6 genetic map contains 67 ESTRFLP and 58 ESTSSR loci with a total map length of 757 cM. Bridging loci permitted the alignment of homologous chromosomes between the parental maps, and a sub-set of genomic DNA-derived SSRs was used to relate linkage groups to the perennial ryegrass reference map. Regions of segregation distortion were identified, in some instances in common with other perennial ryegrass maps. The EST-derived marker-based map provides the basis for in silico comparative genetic mapping, as well as the evaluation of co-location between QTLs and functionally associated genetic loci.An erratum to this article can be found at
M.J. Faville and A.C. Vecchies contributed equally to this work. |
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