Spin-state exchange in fusarium lipoxygenase on binding of linoleic acid. |
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Authors: | Y Matsuda T Beppu K Arima |
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Affiliation: | Department of Agricultural Chemistry, The University of Tokyo, Bunkyo-ku, Tokyo, Japan |
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Abstract: | The electron paramagnetic resonance(EPR) signals of Fusarium lipoxygenase were measured at liquid nitrogen temperature in the presence or absence of substrate, linoleic acid. The spin-state exchange of heme iron in Fusarium lipoxygenase from a low to high spin-state by the addition of linoleic acid was observed. The addition of linoleic acid to the enzyme at pH 9.0 gave rise to the appearance of EPR lines at g=5.92 and 3.58, while at pH 12.0, lines at g=6.12 and 3.41 were newly appeared. At the same time, the resonance at g=4.31 was increased both at pH 9.0 and 12.0 in the presence of linoleic acid. |
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