Streptozotocin toxicity to cultured pancreatic islets of the Syrian hamster |
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Authors: | Peter F Zucker Michael C Archer |
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Institution: | (1) Department of Medical Biophysics, University of Toronto Ontario Cancer Institute, Toronto, Ontario, Canada;(2) Ontario Cancer Institute, 500 Sherbourne Street, M4X 1 K9 Toronto, Ontario, Canada |
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Abstract: | Pancreatic islets of the Syrian golden hamster were maintained in culture for extended periods of time. Toxicity of streptozotocin in these cultures was evaluated by measurement of insulin secretion. Exposure of islets to 1 or 2 mM streptozotocin immediately following isolation resulted in a permanent and dose-related inhibition of insulin secretion. This was accompanied by islet disruption as observed by phase-contrast microscopy. Culture of islets for 24 hours before streptozotocin exposure afforded protection from toxicity. For example, exposure of freshly isolated islets to 2 mM streptozotocin resulted in complete destruction of beta cells, whereas islets similarly exposed after a 24 hr culture period continued to secrete insulin for many months. Islets maintained in culture for one week before exposure to 0.1–0.5 mM streptozotocin, however, became more sensitive than freshly isolated islets. Repeated weekly exposure of cultured islets to a non-toxic concentration (0.1 mM) resulted in sustained suppression of insulin secretion after 11 weeks. |
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Keywords: | pancreatic islets streptozotocin Syrian hamster tissue culture toxicity |
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