The accessibility of phage MS2 RNA to structure specific nucleases in various conditions |
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| Authors: | V V Grechko G I Tymokhina L A Aleshkina J I Kalashnikova S P Majev S K Vassilenko |
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| Institution: | (1) Institute of Molecular Biology, Academy of Sciences of the USSR, Vavilova str. 32, 117984 Moscow, USSR;(2) All-Union Scientific Research Institute of Molecular Biology, Koltzovo, 630090 Novosibirsk, USSR |
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| Abstract: | The accessibility of ds-and ss-segments of phage MS2 RNA to ds-and ss-specific nucleases (RNase III, nuclease SV and nuclease S1) was studied. The results show that the RNA has hydrolysis sites for all the nucleases used. These sites are unvariable in a wide range of the conditions (ionic strength, pH, bivalent cations and temperature) and are not changed also after denaturation-renaturation of the RNA. This testifies that the distribution and interactions of ds-and ss-segments in the whole molecule are very specific and stable. |
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