Immobilization of Candida rugosa lipase on colloidal gas aphrons (CGAs) |
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Authors: | O'Connell P J Varley J |
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Affiliation: | Biotechnology and Biochemical Engineering Group, University of Reading, P.O. Box 226, Reading, RG6 6AP, UK. |
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Abstract: | A novel technique for immobilization of Candida rugosa lipase onto anionic colloidal gas aphrons (CGAs) is described. CGAs are spherical microbubbles (10-100 microm) composed of an inner gas core surrounded by a surfactant shell. In this initial study, greater than 80% lipase (w/w) was effectively retained on the CGAs. Leakage of protein from the CGAs and the activity of the adsorbed lipase decreased with increasing enzyme loading; this indicates that multilayers of lipase may be adsorbing onto the CGAs. The CGA-immobilised lipase displayed normal Michaelis-Menten dependence on substrate concentration and also exhibited greater activity than the free enzyme. |
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Keywords: | Candida rugosa lipase aphrons lipase immobilization lipase adsorption |
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