A novel monoclonal antibody, 1B3.1, binds to a new epitope of the VLA-1 molecule |
| |
| Authors: | I Bank M Hemler M B Brenner D Cohen V Levy J Belko C Crouse L Chess |
| |
| Institution: | Department of Internal Medicine and Oncology, Chaim Sheba Medical Center Tel Hashomer, Israel. |
| |
| Abstract: | A monoclonal antibody, 1B3.1, was raised against a cloned IL-2-dependent T cell line that expresses the T gamma delta T cell receptor. MoAb 1B3.1 reacted with long-term cultured T cell lines of both T gamma delta and T alpha beta lineage, and with in vivo-stimulated T cells, derived from synovial fluid, but not with resting or short-term activated T cells, B cells, or macrophages. Immunoprecipitation of the 1B3.1 target antigens showed that 1B3.1 recognizes a 200/110 kDa molecule that is identical to the VLA-1 heterodimer precipitated by MoAb TS2/7. 1B3.1, however, binds to an epitope of VLA-1 that is distinct from the TS2/7 binding site. This new MoAb could be useful in further studies of the functions of VLA-1, and of the cells that express this molecule. |
| |
| Keywords: | |
|
|
