Physical and functional interactions among basic chromosome organizational features govern early steps of meiotic chiasma formation |
| |
Authors: | Blat Yuval Protacio Reine U Hunter Neil Kleckner Nancy |
| |
Institution: | Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA. |
| |
Abstract: | Analysis of meiotic recombination by functional genomic approaches reveals prominent spatial and functional interactions among diverse organizational determinants. Recombination occurs between chromatin loop sequences; however, these sequences are spatially tethered to underlying chromosome axes via their recombinosomes. Meiotic chromosomal protein, Red1, localizes to chromosome axes; however, Red1 loading is modulated by R/G-bands isochores and thus by bulk chromatin state. Recombination is also modulated by isochore determinants: R-bands differentially favor double-strand break (DSB) formation but disfavor subsequent loading of meiotic RecA homolog, Dmc1. Red1 promotes DSB formation in both R- and G-bands and then promotes Dmc1 loading, specifically counteracting disfavoring R-band effects. These complexities are discussed in the context of chiasma formation as a series of coordinated local changes at the DNA and chromosome-axis levels. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|