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DNA damage, DNA repair and chromosome aberrations of xeroderma pigmentosum cells and controls following exposure to nitrosation products of methylguanidine |
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| Authors: | L.W. Lo H.F. Stich |
| Affiliation: | Cancer Research Centre and Department of Medical Genetics, University of British Columbia, Vancouver, Canada V6T 1W5 |
| Abstract: | Nitrosation of methylguanidine (MG) led to products that caused DNA fragmentation (shift in sedimentation profiles of velocity centrifugation through alkaline sucrose gradients), a DNA repair synthesis (unscheduled uptake of (3H]TdR), chromosome aberrations and a lethal effect of cultured human fibroblasts. The response of repair-deficient xeroderma pigmentosum cells did not differ from that of controls. The nitrosation of MG must be carried out at a pH level below 3, in order to obtain products that react with cellular DNA. The results show that a DNA repair synthesis of human fibroblasts appear to be a sensitive assay for carcinogenic and mutagenic nitrosation products which may be formed within an organism from non-carcinogenic compounds. |
| Keywords: | ADM arginine-deficient medium MEM Eagle's minimum essential medium MG methylguanidine N-MG nitrosation products of methylguanidine MNC methylnitrosocyanamide MNU methylnitrosourea PBS phosphate-buffered saline SDS sodium dodecyl sulphate TdR thymidine XP-E xeroderman pigmentosum from Edmonton |
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